proliferation of muscle satellite cells on intact myofibers in culture

 

 

 

 

Proliferation of muscle satellite cells on intact myofibers in culture (1985) Bischoff Richard. Dynamics of Myoblast Transplantation Reveal a Discrete Minority of Precursors with Stem Cell-like Properties as the Myogenic Source (1999) Jonathan R. Beauchamp et al. Proliferation of muscle satellite cells on intact myofibers in culture. Dev Biol 115:129139.Oxygen-mediated regulation of skeletal muscle satellite cell proliferation and adipogenesis in culture. J Cell Physiol 189:189196. Keywords: Single myofiber cultures, Satellite cells, Muscle injury, Pax7, MyoD.Proliferation of muscle satellite cells on intact myofibers in culture. Whe activated, they are responsible for creating new muscle fibers and/or combining with existing myofibers to facilitate growth and repair.This study sought to accomplish two specific aims, the first of which was to harvest and isolate satellite cells from intact murine muscle, and subsequently grow Myofibers are cultured in dishes coated with Vitrogen collagen, and satellite cells remain associated with the myofibers undergoing proliferation and differentiation on the myofiber surface.Dissociated flexor digitorum brevis myofiber culture system--a more mature muscle culture system. Skeletal muscle satellite cells (SCs) are Pax7 myogenic stem cells that reside between the basal lamina and the plasmalemma of the myofiber.Proliferation of muscle satellite cells on intact myofibers in culture. (1986) Proliferation of muscle satellite cells on intact myofibers in culture.(2011) In vivo tissue engineering of functional skeletal muscle by freshly isolated satellite cells embedded in a photopolymerizable hydrogel. Second, a primary cell tissue culture system was used to test for the presence of activated satellite cells. If there is ongoing myofiber remodelingDev Biol 1993160: 3950.

43. Putman CT, Dusterhoft S, Pette D. Satellite cell proliferation in low frequency-stimulated fast muscle of hypothyroid rat. Proliferation of muscle satellite cells on intact myofibers in culture.Temporal expression of regulatory and structural muscle proteins during myogenesis of satellite cells on isolated adult rat fibers. Dev. Biol. R.

Bischoff, Proliferation of muscle satellite cells on intact myofibers in culture, Developmental Biology, vol. 115, no. 1, pp. 129139, 1986. Myofibers are isolated intact and retain their associated satellite cells. The first protocol discusses myofiber isolation from the flexor digitorum brevis (FDB) muscle.Employing such culture conditions, satellite cells remain associated with the myofibers, undergoing proliferation and Adult muscle stem cells, satellite cells (SCs), endow skeletal muscle with tremendous regenerative capacity.35. Bischoff R (1986) Proliferation of muscle satellite cells on intact myofibers in culture. Morphological analysis of satellite cells in culture and MyHC-2A expression. The morphological aspects of myoblasts cultured in 2 different culture media are shown in Fig.Bischoff R: Proliferation of muscle satellite cells on intact myofibers in culture. Myofibers are isolated intact and retain their associated satellite cells. The first protocol discusses myofiber isolation from the flexor digitorum brevis (FDB) muscle.Employing such culture conditions, satellite cells remain associated with the myofibers, undergoing proliferation and Muscle satellite cells constitute the stem cell pool that sustains and regenerates myofibers in adult skeletal muscle. Here, we show that deletion of Brg1 in primary mouse myoblasts derived from muscle satellite cells cultured ex vivo leads to a cell proliferation defect and apoptosis. Single myofibers can be cultured in standard plating conditions or in floating conditions. Satellite cells on floating myofibers are subjected to virtually no other influence than the myofiber environment.1. Bischoff, R. Proliferation of muscle satellite cells on intact myofibers in culture. Once activated, all satellite cells maintain expression of syndecan-3 and syndecan-4 for at least 96 h, also implicating these HSPGs in muscle regeneration. Inhibition of HSPG sulfation by treatment of intact myofibers with chlorate results in delayed proliferation and altered MyoD expression In this paper we report the proliferation of satellite cells on single muscle fibers isolated from adult rats and placed in culture. Viable fibers were liberated from muscle with collagenase and purified from non-muscle cells. Proliferation of muscle satellite cells on intact myofibers in culture. Ruth Bischoff.Muscle satellite cells are quiescent myogenic stem cells situated between the basal lamina and plasmalemma of mature skeletal (More). Single myofiber isolation and immunofluorescence. Single myofibers were obtained by collagenase digestion of EDL and peroneus muscles, as previously described (Hawke et al.Proliferation of muscle satellite cells on intact myofibers in culture. Proliferation of muscle satellite cells on intact myofibers in culture. Dev. BioI.B) Line graph illustrating the population shift from c-met m-cadherin- (dashed line) to c-met m-cadherin ( solid line) over the first 96 hours in culture of satellite cells on isolated myofibers. Such cultures demonstrated that intact myofibers were a source of proliferative myoblasts, that could subsequently fuse into myotubes (Bischoff, 1975 Konigsberg et al 1975).Am J Anat 106:233245 Bischoff R (1986) Proliferation of muscle satellite cells on intact myofibers in culture.chlorodeoxyuridine [CldU], and iododeoxyuridine [IdU]), and analyzing stem cell progeny during induced regeneration in vivo, we observed extraordinarily high frequencies of segregation of older and younger template strands during a period of proliferative expansion of muscle stem cells. Gillespie, , 2008 Niche regulation of muscle satellite cell self-renewal and differentiation. Cell Stem Cell 2 2231 .Bischoff 1986 (1986) Proliferation of muscle satellite cell on intact myofibers in culture. Myosatellite cells or satellite cells are small multipotent cells with virtually no cytoplasm found in mature muscle. Satellite cells are precursors to skeletal muscle cells, able to give rise to satellite cells or differentiated skeletal muscle cells. Different from the FDB myofibers, the longer EDL myofibers tend to tangle and break when cultured together therefore, EDL myofibers are cultured individually.Bischoff, R. (1986) Proliferation of muscle satellite cells in intact myofibers in culture. Single myofibers can be cultured in standard plating conditions or in floating conditions. Satellite cells on floating myofibers are subjected to virtually no other influence than theReferences 1. Bischoff, R. Proliferation of muscle satellite cells on intact myofibers in culture. Dev. Under normal conditions, satellite cells are retained in a quiescent state and when required, these cells are activated to proliferate and differentiate to repair pre-existing muscle fibers or to a lesser extent fuse with each other to form new myofibers. This chapter describes our laboratory protocols for isolating, culturing, and immunostaining intact myofibers from mouse skeletal muscle as a means for studying satellite cell dynamics.Isolation and culture of skeletal muscle myofibers as a means to analyze satellite cells. In undamaged muscle, satellite cells are a rare, highly dispersed population of mitotically quiescent single cells, maintained in a niche outside the plasmalemma but beneath the basal lamina of host myofibers. Proliferation of muscle satellite cells on intact myofibers in culture.Satellite cells proliferate, differentiate, fuse with existing myofibers, and merge together to form new myofibers (Merly et al 1998). Effect of Added Satellite Cells To determine the effect of added satellite cells on regeneration, cells were obtained from the tibialis muscle of the left leg, expanded in culture, and mixedRec. 180:645-662. Bischoff, R. (1986a) Proliferation of muscle satellite cells on intact myofibers in culture. Muscle growth is generated by satellite cell proliferation, migratory capacity of satellite cells, and fusion to already existent myofibers.Proliferation of muscle satellite cells on intact. skeletal muscles of adult rats results in a reduction of the. myofibers in culture. - DEVELOPMENTAL BIOLOGY 115, 129-139 (1986) Proliferation of Muscle Satellite Cells on Intact Myofibers in Culture RICHARD BISCHOFF Department of Anatomy and za, 10 feb 2018 20:52:00 GMT Proliferation of muscle satellite cells on intact We demonstrate that engraftment of myofiber-associated satellite cells, coupled with an induced muscle injury, markedly alters the environment of young adult host muscle, eliciting a near-lifelong enhancement in muscle mass, stem cell number, and force generation. These data suggested that the presence of multinucleated derivatives of SC-IKK satellite cells may have a negative impact on the proliferation or survivalSingle myofibers and myofiber-associated cells were prepared from two-step collagenase/dispase digestion of intact limb muscles (EDL The Notch pathway is intact in mouse satellite cell: myofiber explant cultures and receptor activation causes increased proliferationIt was noted that satellite cells attached to intact, viable muscle fibers exited quiescence sooner if the culture contained damaged or dead fibers (Bischoff, 1986). These cells can stimulate the proliferation of activated satellite cells by releasing growth factors and cytokines.5.1.2 Fusion between Macrophages and Muscle cells/Myofibers.The entire TA muscle was removed with its tendon insertion on the foot and tibial origin intact. 4. Bischoff, R "Proliferation of muscle satellite cells on intact myofibers in culture," 115 Dev. Biol. 129-139 (1986). 5. Bischoff, R "Tissue culture studies on the origin of myogenic cells during muscle regeneration in the rat," pp. 13-29, in Muscle Regeneration, A. Mauro et al ed Raven Press Bischoff R (1986) Proliferation of muscle satellite cells on intact myofibers in culture. Dev.Biol. 115:129-139.

77. Feldman JL, and Stockdale FE (1991) Skeletal muscle satellite cell diversity: satellite cells form fibers of different types in cell culture. I further examined satellite cell self-renewal using a well-established paradigm involving culture of dissociated myofibers (Halevy et al 2004 Olguin.Of note, satellite cells play pivotal roles in contributing to muscle regeneration and satellite cell activation, proliferation and differentiation are Single myofibers can be cultured in standard plating conditions or in floating conditions. Satellite cells on floating myofibers are subjected to virtually no other influence than the myofiber environment.1. Bischoff, R. Proliferation of muscle satellite cells on intact myofibers in culture. Satellite cells proliferate to provide myonuclei to growing myofibers before becoming quiescent in mature muscle (Schultz et al 1978).Proliferation of muscle satellite cells on intact myofibers in culture. "Proliferation of muscle satellite cells on intact myofibers in culture." Developmental Biology 115(1): 129-139."Oxygen-mediated regulation of skeletal muscle satellite cell proliferation and adipogenesis in culture." Satellite cells proliferate following muscle trauma (Seale, et al 2003) and form new myofibers through a process similar to foetal muscle development (Parker, et al 2003). After several cell divisions Disruption of the myofiber is reflected by increased serum le-vels of muscle proteins such as creatine kinase. This protein is in intact fibers restricted.C-Met expression and mechanical activation of satellite cells on cultured muscle fibers. Background: As the resident stem cells of skeletal muscle, satellite cells are activated by extracellular cues associated with local damage.Results: We analyzed 244 individual fiber-associated satellite cells in time-lapse video from 24 to 48 hours after myofiber harvest. Unlike intact myofibers, collapsed myofibers displayed a marked increase in cellular budding on their periphery after 6 days in culture, which is a hallmark of MPC proliferation.Bischoff R. Proliferation of muscle satellite cells on intact myofibers in culture. The second approach is to isolate the muscle satellite cells from single intact muscle fiber, which can obtain relatively pure muscle satellite cells, and this method has been successfully used in studies of muscleIsolation and culture of skeletal muscle myofibers as a means to analyze satellite cells. The isolated myofibers are intact and retain their associated satellite cells underneath the basal lamina. The first protocol discusses myofiber isolation from the flexor digitorum brevis (FDB) muscle.These myofibers are cultured in dishes coated with Matrigel.

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